Diversity of antigenic determinants of porcine zona pellucida revealed by monoclonal antibodies.
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Date
1985-03
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Abstract
Monoclonal antibodies derived from ten hybrid cell clones, generated against
porcine zona pellucida gave strong immunofluorescence with zona but the pattern varied from
patchy, thin rim to heavy precipitation type of rim. Five of the 6 monoclonals studied
prevented the binding of the porcine epididymal sperm to homologous oocytes in vitro,
whereas the sixth one was partially effective. All of the 6 monoclonale of this batch inhibited the
lysis of zonae by proteolytic enzymes even at dilutions up to 1 × 10–3
. Three of the four
monoclonals prepared in a subsequent batch gave strong immunofluorescent reactions and
had high titres as determined by enzyme immunoassay. These monoclonals did not, however,
protect the zonae against lysis by proteolytic enzymes. These properties are suggestive of the
heterogeneity of the antigenic determinants in zona and emphasize the employment of
appropriate bioassays for screening and selection of bioeffective antibodies.
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Keywords
Prevention of sperm binding, inhibition of proteolytic lysis of zona, immunofluorescence, enzyme immunoassay
Citation
Bamezai A K, Bhattacharya A, Band A H, Suman, Talwar G P. Diversity of antigenic determinants of porcine zona pellucida revealed by monoclonal antibodies. Journal of Biosciences. 1985 Mar; 7(2): 105-113.