On the presence of sulphate in pituitary lutropin.
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Date
1983-12
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Abstract
The presence of sulphate in the carbohydrate of pituitary lutropin from
different species has been investigated using a biosynthetic approach. Pituitaries from
rats, rabbits, goats, and buffaloes were incubated in the presence of 35SO and the
35SO 4
– -labelled proteins in the tissue immunoprecipitated with a well characterized
anti-sheep lutropin serum. The incorporation into immunoreactive lutropin was low in
the case of rat, rabbit and goat pituitaries while, it was considerable in the case of buffalo
pituitaries. Hence further characterization studies were carried out on 35SO -labelled
proteins of buffaloes. The physico-chemical, immunological and biological properties
of radio-labelled buffalo pituitary material were shown to be similar to those of standard
lutropin. In in vitro conditions of incubations, most of the incorporation of 35SO was
observed into tissue lutropin while under similar conditions of incubation, [14C]-amino
acids were found to get incorporated mostly into medium lutropin. The physiologically
specific releasing hormone, lutropin-releasing hormone was found to
stimulate the release of 35SO -labelled lutropin from the rabbit pituitaries into the
medium. These results give indirect evidence that sulphate could be present in pituitary
lutropin.
Description
Keywords
Sulphate incorporation, pituitary hormone, lutropin, gonadotropin, immunoprecipitation
Citation
Arunasmithasri A S, Rajendrakumar T, Muralidhar K. On the presence of sulphate in pituitary lutropin. Journal of Biosciences. 1983 Dec; 5(4): 355-363.