RNA polymerase activity in isolated nuclei of Nicotiana sanderae callus: Characteristics and modulation during differentiation.
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Date
1983-12
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Abstract
Isolated nuclei from differentiating cultures of Nicotiana sanderae showed
increased levels of RNA polymerase activity as compared to the nuclei from callus cultures.
The RNA synthetic activity was dependent on nucleotide triphosphates and
Mg2+ and was destroyed by RNase. Maximum activity was obtained in the presence of
50 mM (NH4)2 SO4 and α-amanitin inhibited 40% and 55% of the activity in the nuclei
from callus and differentiating tissue respectively. The nuclei from differentiating tissue
elicited a 3-fold increase in RNA polymerase I and a 4-fold augmentation in RNA polymerase
II activities.
Description
Keywords
Nicotiana sanderae, RNA polymerase, phytohormones, differentiation
Citation
Asha P K, Shaila M S, Vaidyanathan C S, Ramakrishanan T. RNA polymerase activity in isolated nuclei of Nicotiana sanderae callus: Characteristics and modulation during differentiation. Journal of Biosciences. 1983 Dec; 5(4): 347-353.