Analysis of alphavirus polypeptides by two dimensional polyacrylamide gel electrophoresis.
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Date
1983-09
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Abstract
Semliki Forest, Sindbis and Chikungunya viruses were grown and radiolabeled
with [3H]-amino acids in Vero cells. Analysis of virus infected cell lysates by two
dimensional polyacrylamide gel electrophoresis resulted in detection of polypeptides
of molecular, weights corresponding to those of E1, P62, ns60, ns70/72 for Semliki
Forest virus, the C, E1, 6K, 14Κ, ΡΕ2, P97, ns60, ns82 for Sindbis virus and E1. P62,
P97, ns70/72 for Chikungunya virus. Charge and molecular weight heterogeneity in
the precursor polypeptide P62 of Semliki Forest virus was detected. Structural polypeptides
e.g. E1 and E2 of Semliki Forest virus and C, E1, E2 of Sindbis virus and E1 of
Chikungunya virus were detected when purified radiolabeled virus preparations were
analyzed by two dimensional polyacrylamide gel-electrophoresis. Membrane glycoprotein
Ε1 and E2 of Semliki Forest and Ε1 of Sindbis and Chikungunya viruses exhibited
charge heterogeneity. In contrast to the marked difference in isoelectric points of E1
and E2 of Sindbis virus; E1 and E2 of Semliki Forest virus had almost identical isoelectric
points.
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Keywords
Alphaviruses, membrane glycoproteins, charge heterogeneity, two dimensional protein gels
Citation
Deshmane Satish, Banerjee Kalyan. Analysis of alphavirus polypeptides by two dimensional polyacrylamide gel electrophoresis. Journal of Biosciences. 1983 Sept; 5(3): 189-201.