Effect of sealing on the incorporation of pyrene in goat erythrocyte ghosts – Α fluorescence study.
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Date
1983-06
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Abstract
The incorporation of pyrene within the membrane interior of goat erythrocyte
ghost has been estimated from its fluorescence spectrum. The excimer to monomer
fluorescence intensity ratio of embedded pyrene is a function of the fluidity of its environment
and the magnitude of its incorporation. Our study shows that this ratio is considerably less
(30%) in a pre-sealed ghost than in the non-sealed ghost revealing that the site of
incorporation of the probe is indeed the hydrophobic interior of the membrane; as in the later
case, the probe has access to the membrane interior from both sides of the membrane. Our
study on kinetics of molecular exchange indicates a very fast (of the order of seconds) transfer
rate of pyrene from probed to unprobed erythrocyte ghosts through the aqueous phase rather
than actual fusion of the membranes.
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Keywords
Erythrocyte ghosts, probe incorporation, excimer fluorescence
Citation
Nandy P, Choudhury M Dutta, Chakrabarti P. Effect of sealing on the incorporation of pyrene in goat erythrocyte ghosts – Α fluorescence study. Journal of Biosciences. 1983 Jun; 5(2): 163-166.