Ontogeny of insulin-receptor interaction: correlation with circulatory insulin levels.
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Date
1983-06
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Abstract
Interaction of [125I]-insulin with intact hepatocytes and its correlation with
circulatory insulin level was examined. The hepatocytes from new-born rats bound lowest
amount of [125I]-insulin (1.39±0.41 pM/mg cell protein) when circulatory insulin level was
high (8±1.5 μU/ml). Hepatocytes from 7 day and 21 day old animals demonstrated a more or
less similar relationship, Cells from 31 day old animals exhibited maximum insulin binding,
activity (5.13±0.18.pM/mg cell protein) against a low serum insulin level (4.25±0.25 μU/
ml). Scatchard analysis of insulin binding shows that the affinity is higher in the hepatocytes
from new-born animals than in the hepatocytes of 31 day old animals. Higher binding
observed in the latter case may be due to a greater number of binding sites. Hepatocytes from
one year old rats bound very little insulin (2.50±0.36 pM/mg cell protein) against a high
circulatory insulin level (9.25±0.85 μU/ml). In view of these results, it appears that the
down-regulation hypothesis holds true during ontogeny too.
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Keywords
Ontogeny, insulin receptor, serum insulin, hepatocytes
Citation
Balapure Anil K, Rastogi A K, Sahib M K. Ontogeny of insulin-receptor interaction: correlation with circulatory insulin levels. Journal of Biosciences. 1983 Jun; 5(2): 125-129.