Effect of temperature on endocytosis and degradation of sulphated proteoglycans by cultured skin fibroblasts.
Loading...
Date
1982-12
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Temperature up to 16°C reduced endocytosis of [35S]-proteoglycans by human
skin fibroblasts to less than 15% of that at 37°C. At temperatures between 20-26°C
endocytosis was more than 50%. At temperatures below 26°C, the relative rate of degradation
of endocytosed [35S]-proteoglycans was several fold less than the rate of endocytosis.
Codistribution of endocytosed [35S]-proteoglycans and the lysosomal marker enzyme b-
hexosaminidase upon subcellular fractionation indicated that endocytotic vesicles containing
[35S]-proteoglycans had fused with lysosomes at 37°C and at 16°C. The prolonged
halflives of endocytosed [35S]-proteoglycans at 16-26°C could not be explained merely by a
temperature dependent reduction of catalytic activity of lysosomal enzymes
participating in the degradation of sulphated proteoglycans.
Description
Keywords
Proteoglycan endocytosis, lysosomes, reduced degradation
Citation
Sudhakaran Perumana R, Prinz Roswithaand, Figura Kurt von. Effect of temperature on endocytosis and degradation of sulphated proteoglycans by cultured skin fibroblasts. Journal of Biosciences. 1982 Dec; 4(4): 413-418.