An acid stable trypsin-chymotrypsin inhibitor from horse gram (Dolichos biflorus).
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Date
1982-09
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Abstract
An inhibitor of trypsin and chymotrypsin was purified from horse gram
(Dolichos biflorus) beans. The concentration of the inhibitor which provided total inhibition
was 0.27 μg/μg tryptic enzyme and 0.46 μg/μg chymotryptic enzyme. The inhibitor was stable
at 37°C between pH of 3 to 11 and at 97°C, upto pH 5.0 only. While the activities were rapidly
lost in 0.1Ν NaO H the loss was only 5 0% in 0.1Ν HCl when kept for 2 h at 97°C. On heating at
pH 7.8, it remained stable upto 80°C with a gradual loss in activities at 97°C and a total loss
occurring by autoclaving at 15 psi for 10 min. Reduction of disulphide bonds by 2-mercaptoethanol,
pronase digestion and boiling in the presence of 1 Μ NaCl led to reduction in
the activities. However, the inhibitor was resistant to the action of pepsin and subtilisin and to
urea at 37°C.
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Keywords
Trypsin-chymotrypsin inhibitor, horse gram, protease inhibitor, Dolichos biflorus
Citation
Mehta S L, Simlot M M. An acid stable trypsin-chymotrypsin inhibitor from horse gram (Dolichos biflorus). Journal of Biosciences. 1982 Sept; 4(3): 295-306.