A rapid method for preparation of sarcolemma from frog skeletal muscle.
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Date
1981-09
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Abstract
A rapid method for the preparation of sarcolema from frog skeletal muscle has
been described. The purified cell segments were transparent and devoid of contractile
material. The Na+, Κ+ -ATPase and 5'-nucleotidase activities in sarcolemma purified by
this method were comparable to those reported for sarcolemmal preparations purified by
density gradient centrifugation. The preparation also possessed acid phosphatase, alkaline
phosphatase and Κ+-activated, ouabain-sensitive p-nitrophenyl phosphatase activities. The
cholesterol to phospholipid ratio of the sarcolemma was 0.33, indicating its high purity; further,
the preparation was free from mitochondria and contractile proteins.
Description
Keywords
Skeletal muscle, plasma membrane, sarcolemma
Citation
Zaidi S I M, Pandey R N, Kidwai A M, Murti C R Krishna. A rapid method for preparation of sarcolemma from frog skeletal muscle. Journal of Biosciences. 1981 Sept; 3(3): 293-302.