In vitro and in vivo nitrogenase activity of Rhizobium mutants and their symbiotic effectivity.
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Date
1981-06
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Abstract
A slow growing nitrogen-fixing strain of Vigna radiata var. aureus (mung bean)
Rhizobium which expressed nitrogenase activity in a synthetic medium was isolated from its
native population. Mutants with decreased and increased nitrogenase activity were derived
from this strain by treatment with acridine orange and ethidium bromide. These mutants were
tested for symbiotic effectivity in vivo. The effectivity of mutants with decreased nitrogenase
activity in the culture medium was lower than the parent strain; however, the effectivity of
mutants with higher nitrogenase activity did not increase above that of the parent. This
suggests that the plant is perhaps a limiting factor in the full expression of rhizobial nitrogenase
in the nodules.
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Keywords
Nitrogenase activity, symbiotic effectivity, inoculation, mutant strains, nodulation, nitrogen fixation
Citation
Dadarwal K R, Kundu B S, Tauro P. In vitro and in vivo nitrogenase activity of Rhizobium mutants and their symbiotic effectivity. Journal of Biosciences. 1981 Jun; 3(2): 117-124.