Lipid requirements for axenic cultivation of Entamoeba histolytica.
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Date
1980-09
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Abstract
Fatty acids, cholesterol and glucose present in axenic medium are utilized by growing
Entamoeba histolytica but the amoeba is unable to synthesize cholesterol from [U —14 C — ] glucose
although the label is incorporated into the fatty acids and non-saponifiable fractions of the
organism. Exogenously-added sonicated dispersions of cholesterol, β-sitosterol, lanosterol,
lecithin and lauric, palmitic, linoleic and stearic acids are ingested by the amoebae with subsequent
loss in amoeboid movement. After a few hours the movement is regained. Cholesterol, lecithin
and the fatty acids stimulate amoebic multiplication but are unable to replace serum in the
medium either singly or in combination.
Description
Keywords
Entamoeba histolytica, axenic, cholesterol, lecithin, fatty acids, trophozoite trophozoite multiplication
Citation
Garg N K , Das S R. Lipid requirements for axenic cultivation of Entamoeba histolytica. Journal of Biosciences. 1980 Sept; 2(3): 235-241.