Microbial transformation of isonicotinic acid hydrazide and isonicotinic acid by Sarcina sp.
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Date
1979-06
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Abstract
Metabolism of isonicotinic acid and isoniazid by Sarcina sp. led to the
formation of two metabolites which were characterised as 2-hydroxyisonicotinic
acid and citrazinic acid. The blue pigment formed during fermentation was shown
to be derived from the auto-oxidation of citrazinic acid. 2-Oxo-glutarate accumulated
as the major keto acid when isonicotinic acid or isonicotinic acid hydrazide metabolism
was inhibited by 1 mM sodium arsenite. Isonicotinic acid, 2-hydroxyisonicotinic
acid and 2-oxo-glutarate were oxidised by isonicotinic acid hydrazide
or isonicotinic acid-grown cells; citrazinic acid was, however, not oxidised. Isoniazid
hydrazine hydrolase, isonicotinic acid and 2-hydroxyisonicotinic acid hydroxylases
were detected in the cell-free extract of Sarcina sp. grown on isonicotinic acid
hydrazide or isonicotinic acid.
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Keywords
Microbial transformation, isoniazid, isonicotinic acid, Sarcina
Citation
Gupta R C, Shukla O P. Microbial transformation of isonicotinic acid hydrazide and isonicotinic acid by Sarcina sp. Journal of Biosciences. 1979 Jun; 1(2): 223-234.