Effect of phospholipase-D on rat kidney mitochondria.
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Date
1979-03
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Abstract
Incubation of purified rat kidney mitochondrial fraction with phospholipase-
D resulted in the accumulation of phosphatidic acid in the membrane
due to the degradation of membrane-bound phosphatidylcholine, -serine and
-ethanolamine Simultaneously with the hydrolysis of the phospholipids, cholesterol
and protein were released from the mitochondrial membrane into the medium,
and binding of Ca2+ by mitochondrial membranes increased. Phospholipase Dtreated
mitochondrial fraction exhibited increased swelling in vitro in the early
stages of incubation (15 min) after which the mitochondria were ruptured. Membrane-
bound adenosine triphosphatase was partially inactivated and the enzyme activity
was not significantly restored by incubation with sonicated dispersions of phosphatidylcholine,
-serine and cholesterol. These results indicate that removal of
choline, serine and ethanolamine from membrane-bound phospholipids disrupt
phospholipid-cholesterol and phospholipid-protein association and affect functions
of the membrane.
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Keywords
Mitochondria, phospholipase-D, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, swelling, adenosine triphosphatase, Ca2+ binding
Citation
Zaidi S N A, Shipstone A C, Garg N K. Effect of phospholipase-D on rat kidney mitochondria. Journal of Biosciences. 1979 Mar; 1(1): 75-82.