Studies on plant gums. Role of calcium in polysaccharide-protein interaction in the neem (Azadirachta indica) gum.
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Date
1979-03
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Abstract
The partial removal of tightly bound Ca2+ from dialysed neem (Azadirachta
indica) gum, resulted in the release of a basic protein from a highly anionic
polysaccharide-protein complex as evidenced by chromatographic studies on TEAEcellulose.
Complete removal of Ca2+ caused, in addition, the release of a minor
heteropolysaccharide which was found in association with the basic protein. These
processes were reversed on the addition of Ca2+. The gum, in addition, contained a
protein-rich component accounting for 35% protein and 7ยท5% total carbohydrate.
This component behaved as a distinct entity during ion-exchange chromatography
of the native gum solutions, or which were either partially or completely depleted of
bound Ca2+.
Description
Keywords
Neem, Azadirachta indica gum, calcium, basic protein, polysaccharideprotein interactions
Citation
Nayak B Ramakrishna, Shenoy B C, Pattabiraman T N. Studies on plant gums. Role of calcium in polysaccharide-protein interaction in the neem (Azadirachta indica) gum. Journal of Biosciences. 1979 Mar; 1(1): 61-68.