Mutations at embB306 codon and their association with multidrug resistant M. tuberculosis clinical isolates.
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Date
2015-07
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Abstract
Purpose: The presence of embB306 mutation in ethambutol (EMB)‑susceptible (EMBs) clinical isolates questions
the significance of these mutations in conferring resistance to EMB. The present study was carried out to determine
the occurrence of embB306 mutation in EMB‑resistant (EMBr) and EMBs strains of M. tuberculosis. One hundred
and four multidrug‑resistant tuberculosis (MDR‑TB) strains were also included to establish the relevance of excessive
use of rifampicin (RIF) and isoniazid (INH) in occurrence of embB306 mutations in EMBs M. tuberculosis isolates.
Materials and Methods: Deoxyribonucleic acid (DNA) from M. tuberculosis clinical strains was isolated by
cetyltrimethylammonium bromide (CTAB) method. Phenotypic and genotypic drug susceptibility testing (DST) was
performed on 354 M. tuberculosis isolates by using standard proportion method and multiplex‑allele‑specific polymerase
chain reaction assay, respectively. Results: The overall frequency of embB306 mutations in EMBr isolates was found
to be five times higher than its occurrence in EMB‑susceptible isolates (50% vs 10%). Further, the association between
embB306 mutation and EMB‑resistance was observed to be statistically significant (P = 0.000). Conclusion: The
embB306 is not only the main causative mutation of EMB resistance, but is a sensitive applicant marker for
EMB‑resistance study.
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Keywords
Adaptive mutation, embB306, multidrug resistance, tuberculosis
Citation
Gupta A, Singh S K, Anupurba S. Mutations at embB306 codon and their association with multidrug resistant M. tuberculosis clinical isolates. Indian Journal of Medical Microbiology. 2015 Jul-Sept; 33 (3): 387-392.