Development of touch down‑multiplex PCR for the diagnosis of toxoplasmosis.
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Date
2015-04
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Abstract
Purpose: The diagnosis of toxoplasmosis is challenging since conventional methods like culture and immunofluorescence
are not universally available. Serology, which is used regularly might be negative during early phase of infection
and in immunosuppressed patients or may remain positive for a long time. Several molecular tests have been used
for the diagnosis of toxoplasmosis, but none of them have an internal control which would inform us regarding the
presence of polymerase chain reaction (PCR) inhibitors thus, undermining the confidence of a laboratory physician.
Materials and Methods: We designed a multiplex PCR containing primers targeting human beta globin gene which
would act as internal control and two primers against the B1 gene and 5s gene which aid in sensitive detection of T. gondii.
Results: Multiplex PCR had a sensitivity of 83.3% and specificity of 100%. Conclusion: Multiplex PCR may provide a
sensitive and specific tool for diagnosis of human toxoplasmosis.
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Keywords
Diagnosis, multiplex, polymerase chain reaction, Toxoplasma
Citation
Hallur V, Sehgal R, Khurana S. Development of touch down‑multiplex PCR for the diagnosis of toxoplasmosis. Indian Journal of Medical Microbiology. 2015 Apr-Jun; 33(2): 271-273.