In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast.
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Date
2015-03
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Abstract
Marine yeasts isolated from coastal mangrove ecosystem namely Candida albicans, Kuraishia capsulate and
Sacchromyces cerevisae were screened for the cytotoxicity against a non-transformed Vero (African green
monkey kidney normal cell line) and two cancer cell lines human breast carcinoma cells (HepG2), human breast
carcinoma cells (MCF -7) in different concentrations (1000 to 1.953 μg/ml). Lower doses enhanced the viability
of the cultured cells, MTT assay .higher doses decreased viability of the cells by 50% or more. MTT assay was
used to measure the cell proliferation and survival. Amongst three yeast strains, Sacchromyces cerevisae showed
more than 80% cell viability in Vero cell lines and were studied for further cytotoxicity against HepG2, MCF -7
cell lines respectively.
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Keywords
Yeast, cancer, MTT assay, Cell culture, Vero, HepG2, MCF-7
Citation
Senthilraja P, Kathiresan K. In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast. Journal of Applied Pharmaceutical Science. 2015 Mar; 5(3): 80-84.