Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation.
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Date
2015-03
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Abstract
Collagen is an extracellular matrix protein with great importance in biomedical application. The search for
collagen from various sources is intensified especially from marine source. This study was carried out to extract
collagen from a Malaysian local fresh water fish, Clarias batrachus and characterized its biomedical potential In
vitro. Collagen was extracted from C. batrachus skin using acetic acid method and identified using SDS-PAGE.
MTT assay was performed to determine the effect of coated collagen on cell adhesion and proliferation of L929
skin fibroblast cells. Additionally, scratch assay was performed to examine the effect of collagen coating on
fibroblast cell migration. Result showed that collagen extracted from C. batrachus was made up of collagen type
I, which consists of two α chains (α-1 and α-2) and β chain. At 100 μg/cm2 density, collagen coating significantly
increased fibroblast cell adhesion, proliferation and migration compared to negative control (p< 0.05). As a
conclusion, collagen extracted from C. batrachus increased cell adhesion, proliferation and migration of
fibroblast cells and has potential to be used as an alternative source of collagen.
Description
Keywords
Clarias batrachus, collagen extract, L929 cell, cell migration
Citation
Leong Lek Mun, Sahalan Ahmad Zorin, Tan Liew Huat, Mustafa Nor Hidayah, Rajab Nor Fadilah. Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation. Journal of Applied Pharmaceutical Science. 2015 Mar; 5(3): 19-23.