Cell viability, anti-proliferation and antioxidant activities of Sideritis syriaca, Tanacetum argenteum sub sp. argenteum and Achillea aleppica subsp. zederbaueri on human breast cancer cell line (MCF-7).
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Date
2015-03
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Abstract
The breast cancer is one of the most common types of cancer. Many scientists have focused on the treatment of
this disease for a long time by studying anti-cancer activities of plant extracts as well as synthetics. Lamiaceae
and Asteraceae have been used in anticancer studies due to their phytochemical content. The genus Sideritis,
Achillea and Tanacetumare the members of these families. Sideritis, Achillea and Tanacetum are used as herbal
medication for the treatment ofvariety of diseases. In present study, we demonstrated the biological activity of
Sideritis syriaca (SS), Achillea aleppica (AAZ) and Tanacetum argenteum (TAA) methanol extracts on cell
viability of the breast cancer line MCF7. MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
assay was used to determine the cell viability and proliferation of MCF7 cells. In a dose dependent manner,
methanol extracts (0, 1, 5, 25, 100 and 250 μg/ml) of SS, AZZ and TAA were examined on MCF7, and viability
of cells were determined with MTT staining. Especially, concentrations in 100 and 250 μg/ml of extracts
decreased the cell viability (p<0.001). The results of the current study showed that methanol extracts of SS, AZZ
and TAA effectively inhibited the cell proliferation by decreasing the cell viability of MCF7 cells. Suggesting
that SS, AZZ and TAA can be considered as natural herbal-based anti-cancerous agents.
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Keywords
Cancer cell line, Cell viability, Antiproliferation, Antioxidant, Sideritis, Tanacetum, Achillea
Citation
Yumrutas Onder, Oztuzcu Serdar, Pehlivan Mustafa, Ozturk Nilgun, Poyraz I Eroz, Iğci Yusuf Ziya, Cevik M Ozgur,Bozgeyik Ibrahim, Aksoy Ayse Fatma, Bagıs Haydar, Arslan Ahmet. Cell viability, anti-proliferation and antioxidant activities of Sideritis syriaca, Tanacetum argenteum sub sp. argenteum and Achillea aleppica subsp. zederbaueri on human breast cancer cell line (MCF-7). Journal of Applied Pharmaceutical Science. 2015 Mar; 5(3): 1-5.