In vitro antioxidant activity of the individual herbs of DIA-2, a herbal mixture containing standardized extracts of Allium sativum and Lagerstroemia speciosa.
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Date
2015-02
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Abstract
Hyperglycemia induced oxidative stress is one of the main mechanism involved in the pathogenesis of diabetic
complications. Use of antioxidants as an adjuvant therapeutic approach in diabetes could prevent or delay the
progression of oxidative stress induced diabetic complications. Many herbal drugs possess multiple
pharmacological actions like anti-hyperglycemic, anti-obesity and anti-oxidant properties etc., which could act
through multiple pathways and may offer beneficial effect in the management of multi-factorial diseases such as
diabetes. Development of herbal formulation with ingredients having multiple actions could be a potential
approach for improving therapeutic efficacy. We here report the free radical scavenging activity of a herbal
mixture, DIA-2 and its individual herbs. DIA-2, a herbal formulation containing fixed combination of
standardized aqueous extracts of Allium sativum (ASE) bulbs and Lagerstroemia speciosa (LSE) leaves. The
present study was undertaken to investigate whether a combination of ASE and LSE shows a synergistic
antioxidant effect than its individual herbs. In vitro antioxidant activity for individual herbs and DIA-2 was
determined by DPPH radical scavenging, nitric oxide scavenging assay, total antioxidant capacity, reducing
power assay and ABTS radical scavenging methods respectively. Our results suggest that DIA-2 could
synergistically enhance the antioxidant activity and could be attributed due to the synergistic actions of the
individual ingredients. The data obtained suggests the combined use of ASE and LSE as active ingredients in the
development of antidiabetic herbal formulation, synergizing its therapeutic value in treating hyperglycemia and
associated oxidative stress.
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Keywords
DIA-2, Allium sativum, Lagerstroemia speciosa, Diabetes mellitus, Antioxidant, Oxidative stress
Citation
Kesavanarayanan Krishnan Selvarajan, Kavimani Subramanian, Prathiba Duvuru. In vitro antioxidant activity of the individual herbs of DIA-2, a herbal mixture containing standardized extracts of Allium sativum and Lagerstroemia speciosa. Journal of Applied Pharmaceutical Science. 2015 Feb; 5(2): 22-27.