Antimicrobial and Cytotoxic Activities of Endophytic Fungi Isolated from Artemisia annua L.
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Date
2014-10
Authors
Astuti, Puji
Wahyono
Nuryastuti, Titik
Purwantini, Indah
Purwanto
Journal Title
Journal ISSN
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Abstract
This study was aimed to investigate antimicrobial and cytotoxic effects of endophytic fungi isolated from
A. annua L. The ethyl acetate extracts were obtained by liquid-liquid partition of fermentation broth of
endophytes followed by evaporation. Antimicrobial assay was determined by disc diffusion method. Cytotoxicty
assay was conducted against T47D, WiDr and Vero using MTT assay. Data generated were used to determine
IC50. While DP2 extract did not show any inhibition against all tested bacteria, DP6 extract at 200 μg inhibited
the growth of B. subtilis, P. aeruginosa and S. thypi with the highest inhibition against B. subtilis. Similarly, E2
extract inhibited the growth of B. subtilis, E. coli and S. thypi, with the highest inhibition against B. subtilis. The
three extracts (DP2, DP6 and E2) were found to be cytotoxic against T47D and WiDr with E2 extract to be the
most cytotoxic. These compounds were more selective against T47D cell line with IC50 of 91.7, 74.8 and 12.0
μg/mL, respectively. The IC50 against Vero cell line are 444.4, 140.3 and 87.7 μg/mL, respectively.
Description
Keywords
antimicrobial, cytotoxic, fungal endophytes, A. annua L
Citation
Astuti Puji, Wahyono, Nuryastuti Titik, Purwantini Indah, Purwanto. Antimicrobial and Cytotoxic Activities of Endophytic Fungi Isolated from Artemisia annua L. Journal of Applied Pharmaceutical Science. 2014 Oct; 4(10): 47-50.