Comparison of third generation ELISA and conventional nested RT-PCR for detection of HCV among hemodialysis patients.
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Date
2014-08
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Abstract
Hepatitis C virus is one of the main cause of chronic hepatitis in developing countries. The current study was
done to evaluate the efficacy of the third generation ELISA compared to nested RT- PCR for establishing the
diagnosis of hepatitis C virus (HCV) in patients on hemodialysis. This descriptive, cross-sectional study was
carried out on 237 Hemodialysis patients in Lucknow, Uttar Pradesh. The retrospective demographic data of the
subjects was collected and the patient’s serum samples were analyzed by ELISA & RT-PCR for HCV. In the
present study, of total 21 HCV positive either by ELISA or PCR 12 (57.14%) were positive for both RT-nested
PCR and ELISA. Total four (19.05%) patients were positive for HCV by RT-nested PCR and negative by ELISA
while five (23.81%) patients were negative for RT-nested PCR and positive for ELISA. PCR method is
accredited as a specific and reliable method suitable for screening of HCV and is recommended for establishing
exact and final diagnosis of these patients. However third generation ELISA assays have many advantages in the
diagnostic setting including ease of automation, ease of use, relative cost-effectiveness, and low variability.
Description
Keywords
ELISA, RT-PCR, Hepatitis C virus, hemodialysis
Citation
Prakash Shantanu, Sankhwar S N, Jain Amita, Usman Kausar, Prasad Narayan, Saha D, Singh K P, Parul Jain. Comparison of third generation ELISA and conventional nested RT-PCR for detection of HCV among hemodialysis patients. Journal of Applied Pharmaceutical Science. 2014 Aug; 4(8): 18-22.