Comparative Study of Calix-6-arene, 2-Hydroxy Propyl Beta Cyclodextrin and 18-Crown-6 as Ionophores in Potentiometric Ion-Selective Electrodes for Determination of Trigonelline in Trigonella foenum-graecum Seeds Extract and Plasma.
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Date
2014-07
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Abstract
The present work evaluates the influence of Calix-6-arene, 2-hydroxy propyl β-cyclodextrin and 18-crown-6 as
ionophores on the fabrication of trigonelline selective electrodes 1, 2 and 3, respectively. The three proposed
sensors showed Nernstian slopes of 59, 60 and 55.75 mV/concentration decades over pH range 4-9 for sensors 1,
2 and 3, respectively. Both sensors 1 and 2 covered the concentration range 10-3 – 10-5 M, while sensor 3 showed
linear response over the range 10-2 – 10-4 M. The proposed sensors offer the advantages of fast response and
moderate stability time. The selectivity coefficients of the developed sensors indicated excellent selectivity for
trigonelline. The proposed electrodes were successfully applied for direct determination of trigonelline in pure
form, Trigonella foenum-graecum seeds extract and plasma without prior separation or pretreatment steps. The
proposed sensors can be used in quality control labs and in clinical trails for routine analysis of trigonelline in
Trigonella foenum-graecum seeds extract and plasma.
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Keywords
Ion selective electrodes, ionophores, trigonelline, Trigonella foenum-graecum, plasma
Citation
Ghany Maha F Abdel, Hussein Lobna. A, Yamani Hend Z. Comparative Study of Calix-6-arene, 2-Hydroxy Propyl Beta Cyclodextrin and 18-Crown-6 as Ionophores in Potentiometric Ion-Selective Electrodes for Determination of Trigonelline in Trigonella foenum-graecum Seeds Extract and Plasma. Journal of Applied Pharmaceutical Science. 2014 July; 4(7): 32-37.