Selection and characterization of probiotic lactic acid bacteria with heterocyclic amine binding and nitrosamine degradation properties.
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Date
2014-07
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Abstract
Potential probiotic strain for being health protectant especially intestinal illness is strain specific. This study
investigated the selection of a new strain of probiotic of non-human origin and of human origin with the
properties of intestinal protection against cancer. From the primary screening results, the human feces origin
strains showed more bile salt tolerance than the fermented food origin strains. Whereas none of the human feces
origin isolates could grow well in the acid condition. Lactobacillus plantarum CM4 was the new probiotic of
non-human origin strain for this study. CM4 cells is said to tolerate and grow in 0.3% bile salt after 5 hours of
incubation, at pH3 after 6 hours of incubation. This is in agreement with in vivo study for intestinal adherence
ability of probiotic, a live CM4 cells was able to persist in mice small intestine and colon for 5 days. Live CM4
cells showed most effectiveness to bind 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) mutagen after
24 hours of incubation with 46.32% of binding ability while 144 hours of incubation with 85.34% of binding
ability was the most effective for 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) mutagen. The significant
difference (p<0.05) was found at all those time points. Moreover, the CM4 strain could degrade
diphenylnitrosamine (DPN) better than 1-nitrosopyrrolidine (NPR) with dose response relationship activity.
These imply that the CM4 strain could be the value added for the consuming pharmaceutical probiotic product
based on scientific proof of its role in intestinal survival properties and cancer prevention through binding PhIP
and IQ mutagen as well as degrading nitrosamine.
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Keywords
Probiotic, Lactobacillus, plantarum, heterocyclic amine, nitrosamine, cancer
Citation
Duangjitcharoen Yodsawee, Kantachote Duangporn, Prasitpuripreecha Chutinun, Peerajan Sartjeen, Chaiyasut Chaiyavat. Selection and characterization of probiotic lactic acid bacteria with heterocyclic amine binding and nitrosamine degradation properties. Journal of Applied Pharmaceutical Science. 2014 July; 4(7): 14-23.