Estimation of ursolic acid from Urtica dioica L. using validated HPTLC method.
Loading...
Date
2014-05
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Urtica dioica L. (Urticaceae, leaves) is commonly used in traditional systems of medicine for the treatment of a
wide range of disorders. The present work emphasizes on a validated HPTLC method for estimation of ursolic
acid from U. dioica leaves and its available formulation. Chromatographic separation was achieved on silica gel
60 F254 TLC plate with toluene: ethyl acetate: formic acid (7:3:0.1, v/v/v) as a mobile phase. Detection of ursolic
acid was carried out by derivatizing the plate with Liebermann Burchard reagent at 110°C for 10 min. Camag
TLC scanner 4 equipped with winCATS software was used for densitometric scanning at 366 nm. The accuracy
of the method was checked by conducting various validation parameters according to ICH guidelines. The
method was found applicable to evaluate the impact of regional variation on ursolic acid content in U. dioica
leaves. The research also highlights estimation of ursolic acid from a marketed herbal formulation of U. dioica
leaves. The described HPTLC method was found useful for quantitation of bioactive marker ursolic acid and can
be used as a routine quality control tool for the assessment of botanicals.
Description
Keywords
Geographical variation, HPTLC, leaves, ursolic acid, Urtica dioica
Citation
Shailajan Sunita, Hande Harshada, Singh Dipti, Tiwari Bhavesh. Estimation of ursolic acid from Urtica dioica L. using validated HPTLC method. Journal of Applied Pharmaceutical Science. 2014 May; 4(5): 92-95.