In vitro cytotoxicity effects of single and combination Nigella sativa and Zingiber zerumbet extracts on human myeloid leukemia (HL60) cells and its mode of cell death.
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Date
2014-05
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Abstract
Human myeloid leukemia (HL60) cell line is the precursor and the attractive model of human cell for studying
molecular event drug evaluation and cell differentiation. This research investigate the interactive effects between
Nigella sativa (Ns) and Zingiber zerumbet (Zz) treated on HL60 cell line for 24 hours at non constant ratio of
combination doses. To explain cytotoxicity effect of single and combination extracts is using 3-[45-
dimethylthizol-2-yl]-25-diphenyltetrazolium bromide (MTT) assay. The combination of Ns and Zz at various
combination doses setting showed the antagonism interactive effects. Ns and Zz showed more potent as anti
proliferative agent when treated alone as compared to co-administrations of both. As single agent the IC50 of Ns
(Petroleum ether extract) is 654.9ug/ml which is less cytotoxic as compared to Zz (Hexane extract) that inhibit
50% of HL60 viable cells at 63.72ug/ml (p<0.05). In conclusion combination between Ns and Zz conveyed the
antagonist interactive effects and indicated that Ns and Zz could not be combined together in order to achieve a
safe drug.
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Keywords
Human myeloid leukemia (HL60), Nigella sativa (Ns), Zingiber zerumbet (Zz), 3-[45-dimethylthizol-2-yl]-25-diphenyltetrazolium bromide (MTT), Apoptosis
Citation
Norfazlina M N, Farida Zuraina M Y, Rajab N F, Mohd Nazip S, Rumiza A R, Suziana Zaila C F, Lek Mun L, Nurshahirah N, Florinsiah L. In vitro cytotoxicity effects of single and combination Nigella sativa and Zingiber zerumbet extracts on human myeloid leukemia (HL60) cells and its mode of cell death. Journal of Applied Pharmaceutical Science. 2014 May; 4(5): 51-55.