Characterization of cellulase producing Bacillus and Paenibacillus strains from Thai soils.
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Date
2014-05
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Abstract
Seventeen strains of cellulase producing bacteria were isolated from soil samples collected in Nan province,
Thailand. They exhibited cellulase activity as a clear zone surrounded their colonies grown on carboxymethyl
cellulose (CMC) agar medium ranged from 0.63 to 2.95 cm in diameter. Their hydrolysis capacity values were
1.65 - 7.55. The bacteria isolated were divided into 2 groups and belonged to genus Bacillus and Paenibacillus
based on their phenotypic characteristics and chemotaxonomic characteristics such as meso-diaminopimelic in
cell wall peptidoglycan and menaquinones of MK-7. Bacillus strains, P3-1 and P4-6 in Group I, produced
maximum cellulase at 0.015 U ml-1. Their optimal pH and temperature for enzyme production and enzyme
activity were 7.0 and 50 oC, respectively. The strains P3-1 and P4-6 were closely related to Bacillus velesensis
LMG 22478T (100% similarity) whereas representative strain, S10-4 in Group II, was closely related to P.
cellulositrophicus KCTC 13135T (98.7% similarity) based on 16S rRNA gene sequence. On the basis of their
phenotypic, chemotaxonomic characteristics and phylogenetic analysis of 16S rRNA gene sequence, the strains
P3-1 and P4-6 were identified as B. velesensis and the strain S10-4 was a novel species in the genus
Paenibacillus.
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Keywords
Cellulase, Bacillus, Paenibacillus, 16S rRNA gene sequence, Thai soil
Citation
Akaracharanya Ancharida, Taprig Thanawan, Sitdhipol Jaruwan, Tanasupawat Somboon. Characterization of cellulase producing Bacillus and Paenibacillus strains from Thai soils. Journal of Applied Pharmaceutical Science. 2014 May; 4(5): 6-11.