Phytase production through response surface methodology and molecular characterization of Aspergillus fumigatus NF191.
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Date
2015-06
Journal Title
Journal ISSN
Volume Title
Publisher
NISCAIR-CSIR, India
Abstract
Phytase play an important role in phytic acid catalysis that act as a food inhibitor in cereals. Here, we isolated high phytase producing isolates NF191 closely related to Aspergillus fumigatus sp. from piggery soil. DNA was isolated from the fungal culture and amplified the ITS region using ITS1 and ITS4 primer using PCR. The 400-900 bp amplicon was gel eluted and subjected to sequencing. The sequencing results were assembled and compared with NCBI data base which showed the 99% identity of Aspergilllus fumigatus. Different carbon sources viz., fructose, galactose, lactose, dextrose, sucrose, maltose and different nitrogen sources (organic & inorganic) NH4Cl, NH4NO3, (NH4)2SO4, KNO3, NaNO3, urea, yeast extract, peptone, beef extract were tested for optimal production. The 0.3% dextrose, 0.5% NH4NO3 and 96 h incubation time showed the best production and enzyme activity at 45 ÂșC incubation temperature. The selected parameters, dextrose, ammonium sulphate and incubation time, when employed with statistical optimization approach involving response surface optimization using Box Behnken Design, gave a 1.3 fold increase in phytase production compared to unoptimized condition.
Description
Keywords
Box Behnken Design (BBD), Molecular characterization, Phytic acid, RSM
Citation
Gangoliya Shivraj Singh, Gupta Raj Kishor, Singh Nand Kumar. Phytase production through response surface methodology and molecular characterization of Aspergillus fumigatus NF191. Indian Journal of Experimental Biology. 2015 Jun; 53(6): 350-355.