Production of 4-Ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of Ipomoea batatas Lam.: A Comparative study.
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Date
2015-05
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NISCAIR-CSIR, India
Abstract
A comparative study was done on the production of 4-ipomeanol from root tubers of Ipomoea batatas and its rhizogenic callus. Best callusing response was obtained on MS medium supplemented with 11 µM NAA (α-Naphthaleneacetic acid) and 1 µM KIN (Kinetin). Effect of various elicitors (Fusarium solani, chitin and chitosan) on the production of 4-ipomeanol was studied. Methanol extract of the samples were purified by column chromatography and detected using TLC. Identification of 4-ipomeanol was confirmed using HPLC and quantified spectrophotometrically. A mass spectrum was recorded to confirm the presence of 4-ipomeanol. The calli grown under chitin produced highest (6.61mg g-1) amount of 4-ipomeanol. This is the first report on in vitro production of 4-ipomeanol from I. batatas. Since 4-ipomeanol is reported to be present only in I. batatas, this study would help in standardizing protocols for large scale production without affecting its natural flora.
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Keywords
Callus culture, Chitin, Chitosan, Furanoterpenoid, Sweet potato
Citation
Mohanraj Remya, Subha S. Production of 4-Ipomeanol, an anticancer agent from the root tubers and rhizogenic callus of Ipomoea batatas Lam.: A Comparative study. Indian Journal of Experimental Biology. 2015 May; 53(5): 297-304.