Enhanced Chrysene Degradation by a Mixed culture Biorem-CGBD using Response Surface Design.
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Date
2015-05
Journal Title
Journal ISSN
Volume Title
Publisher
NISCAIR-CSIR, India
Abstract
Degradation of chrysene, a four ringed highly carcinogenic polycyclic aromatic hydrocarbon (PAH) has been demonstrated by bacterial mixed culture Biorem-CGBD comprising Achromobacter xylosoxidans, Pseudomonas sp. and Sphingomonas sp., isolated from crude oil polluted saline sites at Bhavnagar coast, Gujarat, India. A full factorial Central Composite Design (CCD) using Response Surface Methodology (RSM) was applied to construct response surfaces, predicting 41.93% of maximum chrysene degradation with an experimental validation of 66.45% chrysene degradation on 15th day, using a combination of 0.175, 0.175 and 0.385 mL of OD600=1 inoculum of A. xylosoxidans, Pseudomonas sp. and Sphingomonas sp., respectively and a regression coefficient (R2) of 0.9485 indicating reproducibility of the experiment. It was observed that chrysene degradation can be successfully enhanced using RSM, making mixed culture Biorem-CGBD a potential bioremediation target for PAH contaminated saline sites.
Description
Keywords
Biodegradation, Bioremediation, Carcinogenic, Mutagenic, PAH, Polycyclic aromatic hydrocarbons, RSM
Citation
Dave Bharti P, Ghevariya Chirag M, Bhatt Jwalant K, Dudhagara Dushyant R, Rajpara Rahul K. Enhanced Chrysene Degradation by a Mixed culture Biorem-CGBD using Response Surface Design. Indian Journal of Experimental Biology. 2015 May; 53(5): 256-263.