Evaluation of immunostimulatory activity of Chyawanprash using in vitro assays.
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Date
2015-03
Journal Title
Journal ISSN
Volume Title
Publisher
NISCAIR-CSIR, India
Abstract
Chyawanprash is an ayurvedic formulation used in Indian traditional medicinal system for its beneficial effect on human health. We investigated the immunostimulatory effects of Chyawanprash (CHY) using in vitro assays evaluating the secretion of cytokines such as Tumor Necrosis Factor-alpha (TNF-α), Interleukin-1beta (IL-1β) and Macrophage Inflammatory Protein-1-alpha (MIP-1-α) from murine bone marrow derived Dendritic Cells (DC) which play pivotal role in immunostimulation. The effects of CHY on phagocytosis in murine macrophages (RAW264.7) and Natural Killer (NK) cell activity were also investigated. At non-cytotoxic concentrations (20–500 µg/ml), CHY enhanced the secretion of all the three cytokines from DC. CHY also stimulated both, macrophage (RAW264.7) as well as NK cell activity, in vitro. In conclusion, the data substantiates the immunoprotective role of CHY at cellular level mediated by immunostimulation in key immune cells viz. dendritic Cells, macrophages and NK cells.
Description
Keywords
Dendritic cells, Cytokines, Interleukin-1beta, IL-1β, Macrophage inflammatory protein-1-alpha, MIP-1-α, NK cells, Phagocytosis, Tumor necrosis factor-alpha, TNF-α
Citation
Madaan Alka, Kanjilal Satyajyot, Gupta Arun, Sastry J L N, Verma Ritu, Singh Anu T, Jaggi Manu. Evaluation of immunostimulatory activity of Chyawanprash using in vitro assays. Indian Journal of Experimental Biology. 2015 Mar; 53(3): 158-163.