Use of minimally invasive gingival biopsies in the study of inflammatory mediators expression and their correlation with gingival fluid in patients with chronic periodontitis.
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Date
2015-03
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Abstract
Background: There are no studies comparing the gingival crevicular fluid (GCF) cytokines
expression with its corresponding values from the same tissue’s sites. Such comparison might
be of great value since most of the cytokine function is related to cell and/or tissue receptors.
Aims: Our aim was to use minimally invasive biopsies to evaluate the expression of
interferon‑gamma, interleukin 1 (IL‑1) β, IL‑6, IL‑17A, IL‑17F, and their correlation with the
expression in gingival fluid in patients with chronic periodontitis.
Materials and Methods: The collection of gingival fluid comprised 22 samples from 11 patients
(mean age 46.73 ± 10.16 standard deviation years) with chronic periodontitis. The collection
of biopsies comprised 22 samples from the same patients. Gingival fluid and biopsy were taken
from the same site in one shallow and one deep site per patient. Gingival fluid samples were
collected with periopaper® and analyzed using Luminex®. Biopsies were taken with a 2 mm
diameter punch and analyzed for the same mediators using immunohistochemistry.
Results: The gingival fluid showed higher amounts for IL‑1‑β in deep sites. Immunohistochemical
markers were observed in the analyzed cells groups, both in deep and shallow sites, without
significant differences between them. In the comparative analysis between immunohistochemical
markers and GCF, IL‑1‑β showed high concordance in shallow and deep sites.
Conclusions: The use of a standardized punch of 2 mm diameter for periodontal tissue biopsies
seems to be suitable for immunohistochemistry analysis and showed that the GCF may not
express all the markers in the same proportion at the corresponding tissue.
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Keywords
Biopsy, gingival crevicular fluid, inflammation mediators, periodontitis
Citation
Lomba Karina Schittine Bezerra, Beiler Tatiane Flôr Coelho de Souza Breves, Sete Manuela Rubim Camara, Pires Fábio Ramoa, Figueredo Carlos Marcelo da Silva. Use of minimally invasive gingival biopsies in the study of inflammatory mediators expression and their correlation with gingival fluid in patients with chronic periodontitis. Indian Journal of Dental Research. 2015 Mar-Apr; 26(2): 126-130.