Grading angiogenesis in oral squamous cell carcinoma: A histomorphometric study.
Loading...
Date
2015-01
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Context: Like normal tissues, tumors require an adequate supply of oxygen, metabolites and an
effective way to remove waste products. This is achieved by angiogenesis, which is defined as
the process by which new blood vessels are produced by sprouting from preexisting vasculature.
There is a large spectrum of physiological and pathological processes in which angiogenesis
occur, ranging from tissue hypertrophy, wound healing, and inflammation to tumors.
Aims: The present study was designed to morphometrically evaluate the angiogenesis in
different grades of oral squamous cell carcinomas (OSCCs) under light microscope by the use
of H and E stained sections and to assess that whether the parameters of vascularity like mean
vascular density (MVD), mean vascular area (MVA), and total vascular area (TVA) can be used
to histologically grade the tumors.
Subjects and Methods: A total of 10 cases each of well‑, moderately‑ and poorly‑differentiated
SCC cases were retrieved from the archives of the Department of Oral Pathology and Microbiology
and were morphometrically analyzed for mean vascular density (MVD), MVA, and TVA. Ten
cases of normal oral mucosa were taken as Control. Statistical analysis was done using SPSS
19.0 version (IBM, Armonk, NY, USA) software for windows. Group mean for MVD, TVA and
MVA were calculated for 10 cases of each group. “Student’s t‑test” was applied to assess the
intergroup variation of mean values of MVD, TVA, and MVA.
Results: Our results showed significant differences between all the three parameters, that is,
MVD, MVA and TVA when poorly differentiated OSCC was compared with the normal mucosa,
well‑ and moderately‑differentiated OSCC. However, when comparison was made between the
well‑ and moderately‑differentiated OSCC, the differences in the three parameters were present
but not statistically significant.
Conclusion: There was an increased MVD, MVA and TVA in poorly differentiated OSCC, which
could be used as an additional criterion to histologically grade the tumor.
Description
Keywords
Mean vascular area, mean vascular density, oral squamous cell carcinoma, total vascular area
Citation
Wadhwan Vijay, Sharma Preeti, Saxena Chitrapriya, Venkatesh Arvind. Grading angiogenesis in oral squamous cell carcinoma: A histomorphometric study. Indian Journal of Dental Research. 2015 Jan-Feb; 26(1): 26-30