Production of PHB (polyhydroxybutyrate) by rhodopseudomonas palustris KU003 under nitrogen limitation.
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Date
2010-08
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Abstract
A survey of various tannery effluents for the presence of purple non-sulphur
bacteria was undertaken in Warangal district of South India. In all the nine bacterial species,
which included Rhodopseudomonas palustris, R.rutila, R.acdiophila, Rhodopila globiformis,
Rhodospirillum rubrum, Rsp.photometricum, Rhodobacter sphaeroides, Rb.capsulatus,
Rhodobacter sp and Rhodocyclus gelatinosus were isolated. Among these Rhodopseudomonas
palustris KU003 was selected for the production of Polyhydroxybutyrate (PHB). Effect of
nitrogen limitation on the production of PHB was tested. PHB accumulation was more at a
nitrogen limitation of 78 mg/L of ammonium chloride. The maximum PHB produced was 180
mg/L of BP medium containing glucose as carbon source. Significance of the above in the light
of existing literature is discussed in this communication.
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Keywords
Rps.palustris, Polyhydroxybutyrate, nitrogen limitation
Citation
Merugu Ramchander, Girisham S, Reddy S M. Production of PHB (polyhydroxybutyrate) by rhodopseudomonas palustris KU003 under nitrogen limitation. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Aug-Oct; 1(2): 676-678.