The Anticonvulsant Effect of Eupatorium Birmanicum DC Leave Extract Alone and in Combination with Phenytoin Against MES Seizure in Albino Mice.
Date
2014-03
Journal Title
Journal ISSN
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Abstract
To evaluate the anticonvulsant activity of
aqueous extract of Eupatorium birmanicum DC leave (EB)
alone and in combination with phenytoin against MES
seizure in albino mice. Method: Aqueous extract of EB was
prepared using Soxhlet apparatus. The anticonvulsant effect
of the extract was tested on prescreened albino mice at 3
doses (200, 400 & 800 mg/kg). After 1 hr of oral
administration of EB the animals were subjected to MES
seizures by convulsiometer with a current of 45 mA for
0.2 sec via transauricular electrodes and the duration of
the THLE was recorded. Sub-anticonvulsant dose of
phenytoin was also determined and the effect of its
combination with the most effective dose of EB tested.
Results: EB aqueous extract exhibited significant
anticonvulsant activity in the MES model at doses 400 mg/
kg (p<0.01) & 800 mg/kg (p<0.001). This reduction in the
duration of THLE at 800mg/kg of EB was further reduced
significantly (p<0.001) when combined with subanticonvulsant
doses of phenytoin (10mg/kg). Conclusion:
The aqueous extract of E. birmanicum leaves showed
significant anticonvulsant activity in MES seizure model in
albino mice and it significantly increased the anticonvulsant
effect of phenytoin in the same animal model.
Description
Keywords
MES, phenytoin, anticonvulsant, eupatorium birmanicum DC
Citation
Babycha L, Singh Th Imoba, Medhabati M, Joychandra Devi O, Bharati R K, Valte V. The Anticonvulsant Effect of Eupatorium Birmanicum DC Leave Extract Alone and in Combination with Phenytoin Against MES Seizure in Albino Mice. Indian Medical Gazette. 2014 Mar ; 148 (3): 106-110.