Multiplex PCR in diagnosis of M. tuberculosis and M. avium co-infection from lymph node in an AIDS patient.
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Date
2015-02
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Abstract
A 35-year-old, HIV-seropositive male (CD4 count 41 cells/mm3) on highly active antiretroviral ( HAART)
presented with fever and weight loss for 3 months and new skin lesions. He was earlier diagnosed of TB and was on
anti-tubercular therapy (ATT). The retroperitoneal lymph node aspirate showed acid-fast bacilli and epithelioid cell
granulomas; however, cultures remained sterile. A dual infection with Mycobacterium tuberculosis and Mycobacterium
avium was diagnosed with multiplex polymerase chain reaction (MPCR). Clarithromycin was added to ATT, and on
follow-up at 1 and 3 months, the patient responded well. Molecular methods like MPCR should be exploited for routine
diagnosis of high-risk patients
Description
Keywords
Co-infection, multiplex polymerase chain reaction, Mycobacterium avium, Mycobacterium tuberculosis
Citation
Sharma K, Mewara A, Gupta N, Sharma A, Varma S. Multiplex PCR in diagnosis of M. tuberculosis and M. avium co-infection from lymph node in an AIDS patient. Indian Journal of Medical Microbiology. 2015 Feb ; 33 (5_Suppl):s151-153.