The dissemination mode of drug-resistant genes in Enterobacter cloacae.
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Date
2015-02
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Abstract
Background: Enterobacter cloacae (E. cloacae) infection has the highest mortality rate among Enterobacter infections.
This study aimed to determine the prevalence and the transmission route of the class I integron, qnr genes, and CTX-M
ESBLs genes in clinical isolates and to analyse the association between the prevalence of MDR genes and the antibiotic
resistance of E. cloacae. Materials and Methods: The antibiotic susceptibility was tested the agar dilution method.
The class I integron, qnr genes, and CTX-M ESBLs genes were detected by polymerase chain reaction (PCR). The
prevalence data were analysed with the Chi-square test. Results: In the 100 clinical isolates, the class I integron-positive
rate was 65%, with 12% on chromosome, 15% on plasmids and 38% on both. The positive rate of qnr genes was 37%
with plasmid location. The positive rates for qnrA, qnrB and qnrS were 6%, 23% and 8%, respectively. The CTX-M
ESBLs-positive rate was 34%. For CTX-M-1 ESBLs, 15% were on chromosome, 6% on plasmids and 4% on both;
for CTX-M-9 ESBLs, 1% was on chromosome and 7% on plasmid; for CTX-M-25 ESBLs, 3% were on chromosome
and 1% on plasmid. Conclusion: Antibiotic resistance genes may be horizontally and vertically disseminated among
E. cloacae, which helps multidrug-resistant (MDR) strains of E. cloacae to be successful nosocomial agents.
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Keywords
CTX-M ESBLs, CTX-M ESBLs, Enterobacter cloacae, integron, qnr gene
Citation
Liu J, Zeng T, Su G, Lin LY, Zhao Y, Yang WQ, Xie WX, Zhao ZG, Li GM. The dissemination mode of drug-resistant genes in Enterobacter cloacae. Indian Journal of Medical Microbiology. 2015 Feb ; 33 (5_Suppl):s87-92.