Early detection of multi-drug resistance and common mutations in Mycobacterium tuberculosis isolates from Delhi using GenoType MTBDRplus assay.
Loading...
Date
2015-02
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Purpose: There is scarcity of prevalence data of multi-drug–resistant tuberculosis (MDR-TB) data and common
mutations responsible in North India. This study aimed to detect MDR-TB among MDR-TB suspects from Delhi
and mutation patterns using GenoType MTBDRplus assay. Materials and Methods: All MDR suspects in fi ve
districts of New Delhi were referred to the laboratory from 1st October 2011 to 31st December 2012 as per criterion
defi ned by Programmatic Management of Drug Resistant Tuberculosis (PMDT). GenoType MTBDRplus assay was
performed on 2182 samples or cultures and mutations in the rpoB gene for rifampicin (RIF) and katG and inhA genes
for isoniazid (INH) were analyzed. Results: A total of 366 (16.8%) MDR-TB cases were diagnosed. MDR rate was
found to be 32%, 16.6% and 10.2% during criterion A, B and C respectively. The most common mutation detected
for RIF was S531L (59.0%) and for INH was S315T1 (88.3%). Mutations S531L and S315T1 occurred signifi cantly
higher in MDR strains as compared to RIF mono-resistant and INH mono-resistant strains, respectively. Average
laboratory turn-around time (TAT) for dispatch of result to districts for test conducted on samples was 4.4 days.
Conclusion: GenoType MTBDRplus is a useful assay for rapid detection of MDR-TB. The common mutations for RIF
and INH were similar to those seen in other regions. However, mutations determining MDR strains and mono-resistant
strains differed signifi cantly for both RIF and INH.
Description
Keywords
Criterion, line probe assay, MDR suspect, mutation
Citation
Singhal R, Myneedu V P, Arora J, Singh N, Bhalla M, Verma A, Sarin R. Early detection of multi-drug resistance and common mutations in Mycobacterium tuberculosis isolates from Delhi using GenoType MTBDRplus assay. Indian Journal of Medical Microbiology. 2015 Feb ; 33 (5_Suppl):s46-52.