Ethanolic extract of Ficuscarica leave Suppresses Angiogenesis by Regulating VEGF-A and Integrin β3 mRNA Expression in Human umbilical vein endothelial cells.
Loading...
Date
2014-10
Authors
Ghambarali, Zahra
Bidmeshkipouri, Ali
Hassan Akrami, Mehri Azadbakht
Rabzia, Arezo
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background: In the present study, we investigated the anti-angiogenic effects of the ethanol extract of Ficus
carica leave on human umbilical vein endothelial cells (HUVECs).
Methods: HUVECs were used in this study. The cells were cultured in DMEM medium and then incubated
with different concentrations of ethanolic extract of Ficus carica leave (0-25 μg\ml) in the presence or
absence of the extract for 24 hours. Cell viability was analyzed using neutral red assay. Endothelial cell tube
formation was measured with the Matrigel basement membrane matrix. The level of VEGF and Integrin β3
mRNA expression in the HUVECs was measured with reverse-transcription quantitative real-time polymerase
chain reaction (RT-q real time PCR).
Results: We observed that the extract dose dependently inhibited the tube formation of HUVECs. Furthermore,
the extract significantly decreased mRNA expression levels of VEGF-A and Integrin β3 in HUVECs at 20
μg\ml concentration of the extract compared to untreated control cells (P<0.05).
Conclusion: Our findings suggest that ethanolic extract of Ficus carica leave contains anti-angiogenic
activities and could be a candidate as a potential agent for the prevention of angiogenesis related disorders.
Description
Keywords
Citation
Ghambarali Zahra, Bidmeshkipouri Ali, Hassan Akrami Mehri Azadbakht, Rabzia Arezo. Ethanolic extract of Ficuscarica leave Suppresses Angiogenesis by Regulating VEGF-A and Integrin β3 mRNA Expression in Human umbilical vein endothelial cells. Indian Journal of Physiology and Pharmacology. 2014 Oct-Dec ; 58 (4) : 407-415.