Comparison of four diagnostic techniques for detection of Trichomonas vaginalis infection in females attending tertiary care hospital of North India.
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Date
2015-01
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Abstract
Background: Trichomonas vaginalis causes a common sexually transmitted
disease trichomoniasis, which may lead to increased risk of transmission of
human immunodefi ciency virus infection and other pelvic infl ammatory diseases.
Wet mount examination is the most common test for diagnosis, but it has low
sensitivity. Acridine orange staining can be used for diagnosis, but it requires
special microscopic facility. Culture is considered as the gold standard, but it
takes a long time for diagnosis. OSOM Trichomonas Rapid Test is a recently
introduced rapid method based on immunochromatographic assay of trichomonal
protein antigens. Hence, the present study was done to compare these four
diagnostic techniques for detection of trichomoniasis in females with vaginal
discharge. Materials and Methods: Vaginal swabs were taken from 835
female patients and wet mount examination, acridine orange staining, culture
in Kupferberg medium, and OSOM Trichomonas Rapid Test, were performed.
Results: Out of 835 patients included in our study, 68 (8.1%) positive cases
of trichomoniasis were detected by culture. OSOM Trichomonas Rapid Test
detected 63 (7.5%) cases, acridine orange staining detected 53 (6.3%) cases,
whereas, wet mount examination detected only 45 (5.4%) positive cases.
OSOM Trichomonas Rapid Test performed well and showed high sensitivity
and specifi city of 88.2% and 99.6%, respectively. Conclusion: As OSOM
Trichomonas Rapid Test is a point of care test and gave better results than
both wet mount examination and acridine orange staining; it can be used as a
routine test in peripheral areas lacking laboratory facilities.
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Keywords
Acridine orange staining, culture in Kupferberg medium, OSOM Trichomonas Rapid Test, Trichomonas vaginalis, wet mount examination
Citation
Khatoon Razia, Jahan Noor, Ahmad Siraj, Khan Haris Manzoor, Rabbani Tamkin. Comparison of four diagnostic techniques for detection of Trichomonas vaginalis infection in females attending tertiary care hospital of North India. Indian journal of pathology& Microbiology 2015 Jan-Mar 58: (1) 36-39.