Prevalence of genotype D in chronic liver disease patients with occult HBV infection in northern region of India.
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Date
2014-10
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Abstract
Background: Etiology of nearly 30% cases of chronic viral hepatitis remains
undetected. Occult HBV infection (OBI) has emerged as an important clinical
entity in this scenario. Apart from prevalence and clinical outcome of OBI patients
genotype was determined in northern region of India. Materials and Methods:
A total of 847 patients with chronic liver disease (CLD) were screened for
common viral etiologies and others serological markers of HBV. Amplifi cation
of surface, precore and polymerase genes of HBV was performed in patients
negative for other etiologies. Genotyping and sequencing of the precore region
was performed for OBI cases. Results: Twenty-nine (7.61%) cases of OBI were
identifi edof which 9 had chronic liver disease (CHD), 11 liver cirrhosis (LC) and
9 hepatocellular carcinoma (HCC). Majority of OBI cases were detected by
amplifi cation of surface gene 26 (89.6%), followed by pre-core gene 12 (41.3%).
Their liver functions tests were signifi cantly deranged in comparison to overt HBV
cases. IgG anti HBc was present in 8 (27.6%) OBI cases. Mutation was observed
in 8 (32%) in pre-core region at nt. 1896 of overt HBV cases. Genotype D was
the predominant genotype. In conclusion: OBI in our study was characterized
by predominance of genotype D and more severe clinical and biochemical
profi le in comparison to overt HBV. IgG anti HBc positivity could be utilized as
a marker of OBI. We recommend use of sensitive nested PCR for diagnosis of
OBI, amplifying at least surface and precore gene.
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Keywords
CLD, gene amplifi cation, HBV, OBI, surface gene
Citation
Rizvi Meher, Azam Mohd, Sultan Asfia, Shukla Indu, Malik Abida, Ajmal Masihur Reman, Khan Fatima, Sami Hiba. Prevalence of genotype D in chronic liver disease patients with occult HBV infection in northern region of India. Indian Journal of Pathology & Microbiology. 2014 Oct-Dec 57 (4): 537-541.