Genotype MTBDR plus assay for molecular detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis.
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Date
2014-07
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Abstract
Aim: This study was performed for the rapid identification of Mycobacterium
tuberculosis complex and its resistance to rifampicin and isoniazid, directly
from the sputum samples of pulmonary tuberculosis patients. Materials and
Methods: A commercially available genotype MTBDR plus assay was used for
the identification and detection of mutations in Mycobacterial isolates. A total of
100 sputum samples of pulmonary tuberculosis patients were analyzed by using
the genotype MTBDR plus assay. The MTBDR plus assay is designed to detect
the mutations in the hotspot region of rpoB gene, katG and regulatory region of
inhA gene. Results: The genotype MTBDR plus assay detected 22% multidrug
resistant (MDR), 2% rifampicin (RMP) monoresistant and 1% isoniazid (INH)
monoresistant isolates. In 22 MDR isolates, the codons most frequently involved
in RMP-associated mutations were codon 531 (54.55%), 516 (31.82%) and 526
(13.63%), and 90.90% of MDR isolates showed KatG S315T mutations and 9.1%
showed inhA C-15T mutations associated with INH resistance. Conclusion: The
new genotype MTBDR plus assay represents a rapid, reliable tool for the detection
of MDR-TB, wherein results are obtained in 5 h allowing early and appropriate
treatment, which is essential to cut the transmission path and reduce the spread
of MDR-TB. The genotype MTBDR plus assay can readily be included in a routine
laboratory work for the early diagnosis and control of MDR-TB.
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Keywords
MDR-TB, molecular diagnosis, genotype MTBDR plus assay
Citation
Sharma Soniya, Madan Molly, Agrawal Charu, Asthana Ashish K. Genotype MTBDR plus assay for molecular detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis. Indian Journal of Pathology & Microbiology. 2014 Jul-sept 57 (3): 423-426.