Significance of Mycobacterium tuberculosis antigen expression in cerebrospinal fluid monocytes in diagnosing tuberculous meningitis.
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Date
2014-04
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Abstract
Background: The aim of this study was to investigate the signifi cance of positive
expression of Mycobacterium tuberculosis, (MTB) antigen in the cerebrospinal
fl uid (CSF) monocytes in diagnosing tuberculous meningitis (TBM). Materials
and Methods: A total of 50 inpatients of TBM, 30 viral meningitis and 20 healthy
controls were studied at the 1st, 2nd, and 4th week during their treatment course.
Immunohistochemical assay were used to detect early secreted antigenic target
6 (ESAT-6) positive cells, and positive cases were also observed. Results: The
percentage of positive cases and positive cells of ESAT-6 in CSF monocytes
were all higher in the 1st and 2nd week than in the 4th week in TBM patients
(P < 0.01); and percentage of positive cases and positive cells of MTB antigen
in CSF monocytes were higher in TBM patients than in viral meningitis and
health control in the 1st and 2nd week (P < 0.01). The sensitivity was 90% and
the specifi city was 92% in the early stage (within 2 weeks) of TBM. Conclusion:
The positive expression of ESAT-6 in CSF monocytes is helpful for the early
diagnosis of TBM.
Description
Keywords
Diagnosis, early secreted antigenic target 6, Mycobacterium antigen, tuberculosis, tuberculous meningitis
Citation
Song Fu Xia, Sun Xu Wen, Wang Xiao Tong, Nai Yu, Liu Zhu Li. Significance of Mycobacterium tuberculosis antigen expression in cerebrospinal fluid monocytes in diagnosing tuberculous meningitis. Indian Journal of Pathology & Microbiology. 2014 Apr-Jun 57 (2): 265-268.