Association of Epstein Barr virus deoxyribonucleic acid with lung carcinoma.
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Date
2013-10
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Abstract
Context: Lung cancer is the leading cause of cancer death worldwide. In addition
to smoking, a variety of other contributing factors, including viral infection,
have been suggested in tumorigenesis. Epstein Barr virus (EBV), which is
linked to various malignancies, seems to be a good candidate. Aims: The aim
of this study was to investigate the association of EBV with lung carcinomas.
Settings and Design: A total number of 90 formalin fi xed paraffi n embedded
lung tissue samples including 48 cases of lung cancers (18 squamous cell
carcinomas [SCCs], 18 adenocarcinomas and 12 small cell carcinomas) and
42 non-tumoral samples (control group), were retrieved from the pathology
archive. Materials and Methods: Following deoxyribonucleic acid extraction,
polymerase chain reaction (PCR) was performed using an EBV-Eph PCR kit.
The positive cases were studied immunohistochemically for the expression
of EBV-late membrane protein-1 (EBV-LMP-1) in tumoral tissues. Statistical
Analysis Used: The t-test and Fisher exact test were used and P < 0.05 was
considered statistically signifi cant. Results: Five of our cases, including four
SCCs and one adenocarcinoma and two control samples showed a positive
reaction in PCR. All positive tumoral cases showed diffuse staining with LMP-1
in immunohistochemistry. Conclusions: We found a signifi cant difference in
the presence of the EBV genome in cases of lung SCC compared to other lung
lesions (P = 0.02). According to our data, EBV is not at major play in the nonlymphoepithelioma-
like cancers of the lung in general, but may have a role in
the tumorigenesis of some lung SCCs.
Description
Keywords
Epstein Barr virus, lung cancer, polymerase chain reaction
Citation
Jafarian Amir Hossein, Omidi-Ashrafi Abbsali, Mohamadian-Roshan Nema, Karimi-Shahri Mahdi, Ghazvini Kiarash, Boroumand-Noughabi Samaneh. Association of Epstein Barr virus deoxyribonucleic acid with lung carcinoma. Indian Journal of Pathology & Microbiology. 2013 Oct-Dec 56 (4): 359-364.