Speciation of coagulase negative Staphylococcal isolates from clinically significant specimens and their antibiogram.
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Date
2013-07
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Abstract
Background: Despite their frequency as contaminants, coagulase-negative
Staphylococci (CONS) have become important nosocomial pathogens,
accounting for 9% of all nosocomial infections. These infections are diffi cult
to treat because of the risk factors and the multiple drug resistance nature of
these organisms. Materials and Methods: One hundred and two consecutive
CONS were isolated from various clinical samples like blood, pus, urine, urine
catheter tip and gastric lavage. Most of the blood samples were from patients
with risk factors (immunocompromised or on medical devices). After confi rming
the isolates as CONS, species-level identifi cation was performed by simple,
non-expensive conventional methods and antibiotic sensitivity testing was also
carried out. Results: Of 102 CONS isolates, 100 isolates could be identifi ed to
the species level. Among the 100 isolates, epidermidis was the most common
species isolated, seen in 32%, followed by S. hemolyticus (18%), S. lugdunensis
(12%), S. hominis (10%), S. saprophyticus (8%), S. capitis (6%), S. caprae (4%),
S. xylosus (4%), S. cohni and S. warneri (3% each). In the present study, 56%
of the isolates were methicillin-resistant CONS. Most of the isolates showed
resistance to ampicillin and amoxyclav (89% each), followed by ceftriaxone (52%),
cotrimoxazole (46%), cefotaxime (32%), gentamicin (25%), amikacin (21%).
Conclusion: The increased pathogenic potential and multiple-drug resistance
demonstrates the need to adopt simple, reliable and non-expensive methods for
identifying and determining the antibiotic sensitivity of CONS.
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Keywords
Antibiotic sensitivity test, CONS, S. epidermidis
Citation
Usha M G, Shwetha D C, Vishwanath G. Speciation of coagulase negative Staphylococcal isolates from clinically significant specimens and their antibiogram. Indian Journal of Pathology & Microbiology. 2013 Jul-Sept 56 (3): 258-260.