Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis.
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Date
2013-07
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Abstract
Background: The secreted aspartyl proteinases 2 (Sap2) of Candida
albicans (C. albicans) is a potential marker of candididasis. It is a virulence
factor associated with adherence and tissue invasion. Aim: In order to detect
Sap2 in clinical sera, we developed an indirect competitive enzyme-linked
immunosorbent assay (ELISA). Materials and Methods: Polyclonal antibodies
were produced for Sap2 by injecting Sap2 into a New Zealand White inbred
rabbit. They could be used at a dilution exceeding 1:1200 in an indirect ELISA,
and detected Sap2 concentration up to 1 ng/mL. Results: Of the 286 cancer
serum samples tested, 16.8% were found as candidiasis. The test was simple
and economical to perform and had a level of sensitivity for detection of low-titer
positive sera; thus, it may be proven to be of value in epidemiological studies
on candidiasis.
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Keywords
Antibody, cancer, Candida albicans, ELISA, Sap2
Citation
Wang Yicun, Gao Xiang, gang JU Zhi, Liu Jingyuan, Dong Shuai, Wang Li. Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis. Indian Journal of Pathology & Microbiology. 2013 Jul-Sept 56 (3): 243-247.