Molecular and phenotypic expression of decorin as modulator of angiogenesis in human potentially malignant oral lesions and oral squamous cell carcinomas.
Loading...
Date
2013-07
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background: Decorin is an extracellular matrix, multifunctional small proteoglycan
molecule in tumor stroma that has been shown to be modulator of angiogenesis.
No clinical data is available so far on decorin expression and survival outcome
of oral cancer. Aim: The aim of the present study was to examine molecular
and phenotypic expression of two angiogenesis modulators viz. decorin and
vascular endothelial growth factor-A (VEGF-A) in human potentially malignant
oral lesions (PMOLs) and oral squamous cell carcinomas (OSCC) in relation to
clinico-pathological variables and survival outcome. Materials and Methods:
Tissue biopsies were obtained from 72 PMOLs, 108 OSCC and 52 healthy
controls. The PMOLs included cases of leukoplakias and oral submucous fi brosis.
Immunohistochemistry was performed using antibodies against decorin, VEGF-A
and CD-31. Messenger-ribonucleic acid (mRNA) expression was analyzed by
using real-time polymerase chain reaction. Results: Cytoplasmic staining of
decorin was observed in the basal layer of epithelium in 53 (73.61%) cases
of PMOLs and in peritumoral stroma in 55 (50.92%) cases of OSCC. None of
the cases showed nuclear expression of decorin. Decorin expression both at
phenotypic and molecular level was found to be down-regulated from PMOLs to
OSCC. Lymph node metastasis and reduced decorin expression independently
correlated with overall survival in OSCC. VEGF-A expression had no signifi cant
impact on survival outcome. Conclusion: Micro vessel density and VEGF-A
expression were signifi cantly associated with reduced decorin expression in tumor
stroma suggesting, decorin as angiogenic modulator in OSCC. Down-regulation
of decorin expression and the presence of lymph node metastasis were adverse
factor independently affecting overall survival in OSCC.
Description
Keywords
Angiogenesis, decorin, potentially malignant oral lesions, squamous cell carcinoma, vascular endothelial growth factor-A
Citation
Nayak Seema, Goel Madhu Mati, Bhatia Vikram, Chandra Saumya, Makker Annu, Kumar Sandeep, Agrawal Satya Prakash, Mehrotra Divya, Rath Srikanta Kumar. Molecular and phenotypic expression of decorin as modulator of angiogenesis in human potentially malignant oral lesions and oral squamous cell carcinomas. Indian Journal of Pathology & Microbiology. 2013 Jul-Sept 56 (3): 204-210.