Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina.
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Date
2013-12
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Abstract
Background: To observe the impact of application of bio‑amniotic membrane immersed in 5‑fluorouracil
solution in trabeculectomy on the retina in a rabbit model. Materials and Methods: Healthy white
New Zealand rabbits were randomly assigned into three groups with 20 in each group. Bio‑amniotic
membranes of 4 × 5 mm immersed in either physiological saline/water for 10 min, or 25 mg/mL 5‑fluorouracil
solution for 5 and 10 min, respectively, were applied on rabbit eyes during trabeculectomy. At 7, 14, 21, and
28 days of postoperation, five rabbits from each group were examined with electroretinogram (ERG). After
being examined for eye pressure and bleb morphology, rabbits were sacrificed by air embolism and their
retinas were collected and examined by transmission electron microscopy (TEM). In addition, 5‑fluorouracil
amount in bio‑amniotic membranes was measured using high‑performance liquid chromatography.
Results: Each bio‑amniotic membrane could absorb 59.004 μg and 75.828 μg 5‑fluorouracil after being
immersed in 5‑fluorouracil solution for 5 and 10 min, respectively. Application of these bio‑amniotic
membranes in trabeculectomy could promote the formation of well‑functioning bleb and maintain
intraocular pressure, although it had no effect on retina structures as examined with ERG and TEM.
Conclusion: Application of 5‑FU soaked bio‑amniotic membrane in rabbit eye trabeculectomy is effective
and safe.
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Keywords
Biological amniotic membrane, 5‑fluorouracil, retina, trabeculectomy
Citation
Zhang Chenming, Wu Xinyi, Liu Wei, Wang Jianrong, Guo Bailin, Sun Yan, Zhang Yuguang, Zhang Hua, Wang Yu, Zhao Jun. Impact of application of bio-amniotic membrane immersed in 5-fluorouracil solution in trabeculectomy on rabbit retina. Indian Journal of Ophthalmology. 2013 Dec ; 61 (12): 734-738.