Cytokine gene polymorphism (interleukin-1β +3954, Interleukin-6 [−597/−174] and tumor necrosis factor-α −308) in chronic periodontitis with and without type 2 diabetes mellitus.
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Date
2014-05
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Abstract
Background: Pro-inflammatory cytokine gene polymorphisms are potential candidates for
susceptibility for both type 2 diabetes mellitus (DM) and chronic periodontitis (CHP). This study
explored the association of interleukin‑1 beta (IL‑1 β) +3954, interleukin‑6 (IL‑6) −597/−174
and tumor necrosis factor‑alpha (TNF‑α) −308 single nucleotide polymorphisms in CHP with
and without type 2 DM in Malayalam speaking subjects of Dravidian ethnicity.
Materials and Methods: This case control study consisted of 51 chronic periodontitis with
type 2 diabetes mellitus (CHPDM) and 51 CHP patients as cases and 51 healthy subjects as
controls. Polymorphisms were identified by polymerase chain reaction amplification followed
by restriction enzyme digestion and gel electrophoresis.
Results: IL‑1 β (+3954) TT genotype and T allele were significantly associated with CHPDM
group when compared with CHP (P = 0.001), whereas CC genotype and allele C was higher
in CHP subjects (P = 0.001). For IL‑6 (−597) frequency of genotype GA/AA (P = 0.04)
and allele A (P = 0.01) was lower in CHPDM group, and for TNF‑α −308 the frequency of
genotype GA (P = 0.01) and allele A (P = 0.01) was higher in CHP subjects when compared
with controls.
Conclusions: In Malayalam speaking Dravidian population, IL‑6 (−597) genotype GA/AA and
allele A appears to be protective for CHP with type 2 DM. Allele C of IL‑1 β +3954 and allele
A of TNF‑α −308 appears to be risk factors for CHP individuals.
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Keywords
Cytokines, periodontitis, polymorphism, single nucleotide, type 2 diabetes mellitus
Citation
Sharma Nitin, Joseph Rosamma, Arun R, Chandni R, Srinivas K Lekshmy, Banerjee Moinak. Cytokine gene polymorphism (interleukin-1β +3954, Interleukin-6 [−597/−174] and tumor necrosis factor-α −308) in chronic periodontitis with and without type 2 diabetes mellitus. Indian Journal of Dental Research. 2014 May-Jun; 25(3): 375-380.