Influence of L-arginine during bovine in vitro fertilization.
Loading...
Date
2014-12
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The objective of this work was to evaluate the effect of using L-arginine during in vitro fertilization (IVF) on in vitro embryonic development using Bos taurus and Bos indicus semen. Effect of different concentrations (0, 1, 10 and 50 mM) of L-arginine, added to the IVF medium, was evaluated on the fertilization rate at 18 h post-fertilization (hpf), NO3-/NO2- production during IVF by the Griess colorimetric method (30 hpf), cleavage and blastocyst rates (on Day 2 and Day 7 of culture, respectively) and total blastocyst cell number (Day 7 of culture). The results reveal that the addition of 50 mM L-arginine to IVF medium, with either Bos taurus or Bos indicus spermatozoa, decreased the cleavage rate and blastocyst rate compared to the control group. Other concentrations did not affect embryo production. However, 1 mM L-arginine with Bos indicus semen increased the proportion of hatched blastocysts. These results indicate that high L-arginine concentrations may exhibit toxic effects on bovine gametes during in vitro fertilization.
Description
Keywords
Blastocysts, Bos indicus, Bos taurus, Cleavage, Embryo, IVF, Oocytes, Semen, Sperm capacitation, Spermatozoa
Citation
Guimarães Silva, Thiago Velasco, da Silva, Bruno Baraúna, de Sá, André Luiz Alves, da Costa, Nathalia Nogueira, Sampaio, Rafael Vilar , Cordeiro, Marcela da Silva, Santana, Priscila Di Paula Bessa, Adona, Paulo Roberto, Santos, Simone do Socorro Damasceno, Miranda, Moysés dos Santos, Ohashi, Otávio Mitio. Influence of L-arginine during bovine in vitro fertilization. Indian Journal of Experimental Biology. 2014 Dec; 52(12): 1159-1164.